Accurate96-x4 amplifier for Real-Time PCR research

The precise quantitative real-time PCR system is based on Fresnel lens optical signal collection technology, time-adjusted signal separation technology and unique temperature control technology. It achieves an international progressive level of sensitivity, multi-color transition, temperature uniformity and accuracy. The device has 4 or 6 detection channels and is suitable for multiplex PCR applications.

They can be applied to various areas of fundamental research in medicine and biology.

At the moment, the device supports all basic methods of real-time PCR analysis:

– relative quantitative determination;

- absolute quantitative determination;

– allelic discrimination analysis;

– genotyping and gradient PCR, etc.

Sample capacity - 0.1 ml PCR tube × 96, 8 × 12 PCR plate or 96 well plate

Aspiration volume - 10-50 μl

Thermostat - Peltier element

Max. heating / cooling rate - 6.0°C/s

The range of heating temperatures is 4-100°C

Temperature accuracy - ± 0.2°C

Temperature uniformity - ± 0.2 ° C to 95 ° C

The temperature gradient setting range is 30-100°C

The range of setting the difference in temperature gradients is 1 – 36°C

The source of light excitation is 4/6 high-efficiency monochrome LEDs

Detection device - Photomultiplier

Detection mode - Technology of signal separation by time

Excitation / detection wavelength range - 455-650 nm / 510-715 nm

Fluorescent channels - 4/6 channels

Supported Dye - FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ

Sensitivity - One copy of the target DNA

Allowance - 1.33-fold difference in copy number can be isolated in single-stranded qPCR

Dynamic range - 10 orders of magnitude

Features - Absolute quantification, melting curve analysis

Data Summary - Original file, Excel data file. Results include launch parameters, graphical and tabular data

Size [W × D × H] - 50 × 36 × 38 cm

Weight - 25 kg