Densitometer DEN-1

Used to determine the concentration of cells in the fermentation process, to measure optical density at a fixed wavelength and to quantify the concentration of the dissolved substance.

DEN-1 Densitometer (device for turbidity determination in bacterial suspensions) The device determines the concentration of cells in bacterial and microbiological suspensions, using a range of 0.3 to 5.0 McFarland units, which is 100×10^6 to 150×10^7 cells/ml in a quantitative ratio. If necessary, it is possible to study the turbidity level of the solution in a wider range (from 5.0 to 15.0 McFarland units), but the standard deviations of the obtained data may exceed the accepted norms. The DEN-1 densitometer is effective during the research of fermentation processes, sensitization of microorganisms to new antibiotics, as well as determination of bacterial strains in different test systems. In addition, the capabilities of the device allow to measure the optical density of solutions at a fixed wavelength and conduct quantitative analysis of dissolved substances.

The principle of operation consists in obtaining data on the optical density of a particular solution and their subsequent conversion into McFarland units. The instrument is delivered already calibrated and is capable to save the calibration data at repeated switching on and off, however individual calibration of the system on 2-6 points with preservation of the abovementioned limits for McFarland units (from 0.5 to 5.0) is possible. Commercially available standardized solutions such as bioMerieux (France) or Lachema (Czech Republic) can be used for this purpose, as well as those prepared in-house by the laboratory.

If necessary, a battery-operated version can be ordered with an accuracy of 0.01 McFU.