Enzymes

Description

Study of enzyme activity

Enzymes are specific highly effective biological catalysts that accelerate chemical reactions in living systems, synthesized by living cells.

In a living organism, enzymes play an extremely important role, because they participate in all chemical processes that occur both in a single cell and in the organism as a whole.

Determination of enzyme activity:

• allows judging the functional state of many organs and systems for which certain enzymes are characteristic;

• is an indispensable tool in the diagnosis and monitoring of a number of diseases.

Set name	Indicator, method
Alpha-Amylase-SPL	Kinetic, monoreagent
Alpha-Amylase according to Caraway-SPL	Colorimetric, amyloclastic method, according to Karavey
ALT Kin-SpL	UV, kinetic, bireagent
ALT - SpL	Colorimetric, end point, according to Reitman-Frenkel
ALT kin-SPL	UV, kinetic, bireagent
AST - SpL	Colorimetric, end point, according to Reitman-Frenkel
GGT-SpL	kinetic, bireagent
Lipase-SpL	Kinetic colorimetric, liquid
LDH-SpL	UV, kinetic, bireagent
Alkaline phosphatase-SPL	UV, Kinetic, with butyrylthiocholine, bireagent
Cholinesterase-kin SpL	UV, Kinetic, with butyrylthiocholine, bireagent
Acid phosphatase-SpL	UV, alpha-naphthyl phosphate, kinetic
Creatine kinase kin-SPL	UV, kinetic, bireagent
Creatine kinase MV-kin-SPL	UV, kinetic. To determine the MV fraction of creatine kinase