Rebiger paint, 100 ml

Staining of microorganisms is the most common set of methods and techniques in microbiology, used for the detection and identification of microorganisms using a microscope. In their native (natural) state, bacteria have the same refractive index as glass, so they are invisible under a microscope. Staining of microorganisms makes it possible to study the morphological features of microbes, and sometimes to accurately determine their appearance.

Morphological features primarily mean the presence/absence of a capsule formed, for example, by pneumococci and klebmells. A capsule is a mucous formation firmly connected to the cell wall, which has clearly defined outer boundaries. The capsule is different in smears of pathological material. In pure cultures, the capsule is formed less often. The capsule is hydrophilic, it prevents phagocytosis of bacteria. Many bacteria form a microcapsule — a mucous membrane that is removed during an electron microscope. It is necessary to distinguish mucus from the capsule - mucoid exopolysaccharides, which do not have clear outer boundaries. Capsule and mucus protect bacteria from damage and drying, because they are hydrophilic and bind water well; interfere with the action of the protective factors of the macroorganism and bacteriophages. It is detected during special staining methods, which create an unfavorable contrast of the substance of the capsule, because the capsule itself remains unpainted, colorless (detection of capsules with Burri, according to Burri - Gins, according to Rebiger, etc.).

Using:

Smears are painted and fixed at the same time. Non-fixed smears are painted for 15-20 seconds, quickly washed with water and dried with filter paper. During staining with Rebiger's solution, Siberian cut bacilli are stained dark purple, and capsules are stained red-violet.

Packaging: Glass bottles of 100 ml.